Table 2. Serpin cleavage sites determined by MALDI-TOF-MS.

Serpin	m/z determined	Theoretical mass	Deviation (ppm)	Sequence	Position
α1-PI	4133.333	4133.234	+24	380IPM-SIP385	Reactive bond
α1-AC	4623.419	4623.495	−16	381TLL-SAL386	Reactive bond
AGT	4299.351	4299.293	+14	444QQL-NKP449	Reactive center loop
α2-AP	2181.123	2181.097	+12	45SPL-TLL50	N-terminal extension
α2-AP	3489.789	3489.788	<1	458QSL-KGF463	C-terminal extension
α2-AP	3602.870	3602.872	−1	457LQS-LKG462	C-terminal extension
α2-AP	5308.3 (average)	5307.9 (average)	+75	442REL-KEQ447	C-terminal extension

Given are masses determined by MALDI-TOF-MS directly after incubation of serpin with EspPα, theoretical masses, mass deviation, according sequence, and position inside the serpin sequence. Numeration is according to the serpin precursor.