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. 2014 Oct 27;9(10):e110722. doi: 10.1371/journal.pone.0110722

Figure 2. MSC immunophenotyping by flow cytometry.

Figure 2

MSC were analyzed from passages 3 to 5, after being frozen and thawed. (A) Forward Scatter (FSC) and Side Scatter (SC) profiles of MSC. The gate in (A) was drawn to exclude doublets of cells. Ninety-five percent of the analyzed cells were positive for CD90 and CD29 (B), 99.8% were negative for CD45 and CD11b-c (C), 99.8% were negative for CD34, and 99.6% were negative for CD45 (D). These results are consistent with the phenotype of multipotent mesenchymal stem cells.