Figure 2. Effect of temperature on the conformation and antifungal activity of Mo-CBP₃.

(A) Far-UV CD spectra of Mo-CBP₃ (2.22 mM) at various temperatures. (B) CD spectra of Mo-CBP₃ (2.22 mM) after heating at 100°C for 60 min. (C) Light micrographs of F. solani spores in either the culture medium (control) or incubated with Mo-CBP₃ (0.1 mg.mL⁻¹) and either unheated or previously heated at 100°C for 60 min in a water bath. Trials were conducted for 24 h at 22°C in the dark. Bars: 2.5 µm.