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. 2014 Oct 27;9(10):e111448. doi: 10.1371/journal.pone.0111448

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) GAPDH protein levels in the mitochondrial fraction of hearts subjected to control or an IPC-I/R protocol were assessed via liquid chromatography-tandem mass spectrometry, followed by label-free peptide quantification (*p<0.05 vs. control, n = 3). (B) Total GAPDH protein levels in hearts subjected to control or an IPC-I/R protocol were assessed via western blot analysis. Representative western blots are shown for total GAPDH (upper) and enolase (lower) in whole heart homogenates together with the densitometry of GAPDH normalized to enolase (n = 3).