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. 2014 Oct 27;9(10):e111448. doi: 10.1371/journal.pone.0111448

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A representative gel is shown for mitochondrial SNO levels measured via DyLight800 fluorescence after the addition of purified GAPDH or SNO-GAPDH. MW: molecular weight marker; GSNO (+): GSNO treatment was used as a positive control for mitochondrial SNO; Control: non-treated mitochondrial control; GSNO: filtered GSNO treated mitochondria; GAPDH: purified GAPDH treated mitochondria; SNO-GAPDH: SNO-GAPDH treated mitochondria. Please note: the filtration procedure serves to remove excess GSNO following the incubation of purified GAPDH with GSNO (n = 3).