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. 2014 Oct 27;9(10):e111448. doi: 10.1371/journal.pone.0111448

Table 2. SNO proteins from HepG2 cells as identified via SNO-RAC proteomic analysis.

Control GADPH Knock-down GAPDHWT Overexpression GAPDHC150S Overexpression
Dicarbonyl reductase (C212) Dicarbonyl reductase (C212) Dicarbonyl reductase (C212) Dicarbonyl reductase (C212)
60 kDa heat shock protein (C442) 60 kDa heat shock protein (C442) 60 kDa heat shock protein (C442)
Dehydrogenase/reductase SDR family member 1 (C10) Dehydrogenase/reductase SDR family member 1 (C10)
Voltage-dependent anion selective channel protein 1 (C232) Voltage-dependent anion-selective channel protein 1 (C232)
Dynein light chain 1 (C24) Dynein light chain 1 (C24) Dynein light chain 1 (C24)
Voltage-dependent anion selective channel protein 2 (C47) Voltage-dependent anion-selective channel protein 2 (C47)
Long-chain-fatty-acid – CoA ligase 3 (C503) Long-chain-fatty-acid – CoA ligase 3 (C503)
Long-chain-fatty-acid – CoA ligase 4 (C503) Long-chain-fatty-acid – CoA ligase 4 (C503)
Acetyl-CoA acetyltransferase (C119)
Leucine-rich PPR motif-containing protein (C208)
Pyrroline-5-carboxylate reductase 1 (C120)

LC-MS/MS-derived protein identifications from SNO-RAC analysis of HepG2 cell lysate. Each protein identification listed in the table was observed in at least one of six samples with at least two peptide identifications; the cysteine contained in parentheses represents the SNO modified residue; (−) indicates that SNO was not detected for a given protein identification under the specified condition (n = 6).