Table 2. SNO proteins from HepG2 cells as identified via SNO-RAC proteomic analysis.
Control | GADPH Knock-down | GAPDHWT Overexpression | GAPDHC150S Overexpression |
Dicarbonyl reductase (C212) | Dicarbonyl reductase (C212) | Dicarbonyl reductase (C212) | Dicarbonyl reductase (C212) |
60 kDa heat shock protein (C442) | – | 60 kDa heat shock protein (C442) | 60 kDa heat shock protein (C442) |
Dehydrogenase/reductase SDR family member 1 (C10) | – | Dehydrogenase/reductase SDR family member 1 (C10) | – |
Voltage-dependent anion selective channel protein 1 (C232) | – | Voltage-dependent anion-selective channel protein 1 (C232) | – |
Dynein light chain 1 (C24) | Dynein light chain 1 (C24) | Dynein light chain 1 (C24) | – |
Voltage-dependent anion selective channel protein 2 (C47) | Voltage-dependent anion-selective channel protein 2 (C47) | – | – |
– | – | Long-chain-fatty-acid – CoA ligase 3 (C503) | Long-chain-fatty-acid – CoA ligase 3 (C503) |
– | – | Long-chain-fatty-acid – CoA ligase 4 (C503) | Long-chain-fatty-acid – CoA ligase 4 (C503) |
– | – | Acetyl-CoA acetyltransferase (C119) | – |
Leucine-rich PPR motif-containing protein (C208) | – | – | – |
Pyrroline-5-carboxylate reductase 1 (C120) | – | – | – |
LC-MS/MS-derived protein identifications from SNO-RAC analysis of HepG2 cell lysate. Each protein identification listed in the table was observed in at least one of six samples with at least two peptide identifications; the cysteine contained in parentheses represents the SNO modified residue; (−) indicates that SNO was not detected for a given protein identification under the specified condition (n = 6).