Fig. 3.

Effect of heparin on cellular uptake activity of AG73–PEG on the cancer cells 293T-Syn2 (A) and colon26 (B).

The cells were treated with AG73–PEG ([Dox]=20 μg/ml) and heparin (10 μg/ml) or only AG73–Dox for 1 h at 37 °C. After incubation, the cells were washed, and their fluorescence intensities were measured by flow cytometry.