Cytotoxicity of Dox-encapsulating liposomes for 293T-Syn2 (A) or colon26 (B).
The cells were incubated with serial concentrations of Dox–PEG, AG73–Dox, AG73T–Dox, or free Dox for 4 h at 37 °C. After incubation, the cancer cells were washed, and fresh medium was added. The cells were seeded and further incubated for 48 h at 37 °C before the WST assay. †p<0.05; ‡p<0.01; *p<0.005. Data are represented as mean±SD.