Fig. 6.
Confocal microscopy images of 293T-Syn2 after incubation with AG73–Dox. 293T-Syn2 (2×105/well) were seeded in a 24-well plate.
293T-Syn2 cells after 4 h of incubation ([Dox]=10 μg/ml) at 37 °C were washed and transferred into fresh medium for further incubation. The confocal microscopy images were taken after washing. The post-incubation times (0, 30 min, 2, 24, or 48 h) are also indicated above the figure. The cells were fixed and treated with DAPI (blue) for nuclear staining. Blue: fluorescence of DAPI. Red: fluorescence of Dox. Pink: the merged blue and red that indicates the colocalization of DAPI and Dox. The arrows indicate fragmentation of the nuclei. Scale bars represent 20 μm.