Fig. 5.
The ex vivo antineoplastic effect of M1 depends on ZAP deficiency. (A and B) Ex vivo antitumor effect of M1 on clinical tumor explants. Surgical (A) liver and (B) colon cancer specimens were divided into ∼1-mm3 particles and treated with M1 (2 × 107 pfu), vehicle, or HgCl2. Tissue viability was assessed by TECIA after MTT staining. (C) ZAP mRNA expression (normalized to the expression of β-actin) in parallel samples from A and B. Box-and-whisker plots showing median (horizontal line), interquartile range (box), and maximum/minimum range (whiskers) of the data. Resistant, M1-induced inhibition ≤ 10% (n = 12); Sensitive, M1-induced inhibition > 10% (n = 17).