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Loss of ERRα enhances RhoA protein stability. (A) Expression of activated (GTP bound) RhoA and Rac1 in siRNA-treated cells examined by immunoprecipitation. Total RhoA, Rac1, ERRα, and hsp90, were also analyzed in cell lysates before immunoprecipitation. (B) RhoA expression in siRNA-transfected cells treated with cycloheximide (CHX) or MG132. Quantifications (absolute for GTP-bound proteins, relative to hsp90 levels for total proteins) are relative to control conditions.
