Fig. 5.

Effect of furin inhibition on MERS-CoV S infection. Huh-7, MRC-5, WI-38, Vero, and primary NHBE cells were pretreated with increasing concentrations of dec-RVKR-CMK for 2 h. Cells were infected with MERS-CoV at a multiplicity of infection (m.o.i.) of 10 for 8 h (24 h for NHBE). Cells were fixed and immunolabeled for MERS-CoV S and stained for nuclei (DAPI). For each condition, 10× objective fields were randomly acquired and analyzed for total number of cells (DAPI nuclei stain) and S-positive cells (infected cells), with a minimum of 2,400 cells analyzed for each condition. Results are expressed as percentage infected cells, with error bars representing SD from the mean (n = 5). Data were analyzed using one-way ANOVA test.