Fig. 6.

Unc5B is involved in TC axon branching by being expressed in the thalamus. (A) Expression pattern of unc5b was examined at P7 and P14 by in situ hybridization and compared with the distribution of thalamic nuclei as shown by Nissl staining. (Scale bar: 1 mm.) (B and C) Typical examples of TC axons transfected with control shRNA (B) and with unc5b shRNA (C). (D) TC axons cotransfected with unc5b shRNA and shRNA-resistant unc5b (runc5b). (E) Quantification of branching points in B–D and TC axons transfected with dcc shRNA. vLGN, the ventral part of the LGN. *P < 0.01, Dunnett test.