Table 1.
Cephalothin-resistance genes identified in clones of functional metagenomic libraries built in this study
| Gene designation | GenBank accession no. | Library | Cephalothin MIC, µg/mL | Length, aa | e-value | % ID | Accession no. of closest match | Closest match (BLASTX) | |
| blaCEP-01 | KM113767 | MAN_uncultured | 64 | 284 | 0 | 99 | WP_019542800 | β-lactamase (Selenomonas bovis) | |
| blaCEP-02 | KM113768 | B04_cultured | 256 | 316 | 0 | 100 | WP_016087836 | β-lactamase 3 (Bacillus cereus) | |
| blaCEP-03 | KM113769 | MAN_uncultured | 64 | 455 | 0 | 68 | WP_005841913 | β-N-acetyl-glucosaminidase (Bacteroides vulgatus) | |
| blaCEP-04 | KM113770 | B06_cultured | 512 | 385 | 0 | 96 | WP_020798140 | β-lactamase class C [Pseudomonas sp. G5(2012)] | |
| blaCEP-05 | KM113771 | B04_cultured | 256 | 377 | 0 | 91 | ACH58999 | LRA-10 (uncultured bacterium BLR10) | |
| B06_cultured | |||||||||
| B07_cultured | |||||||||
| blaCEP-06 | KM113772 | B09_cultured | 256 | 280 | 7E-173 | 82 | BAL14456 | Metallo-β-lactamase (Serratia marcescens) | |
| blaCEP-07 | KM113773 | B09_cultured | 256 | 288 | 3E-158 | 95 | ABK64020 | Metallo-β-lactamase (Janthinobacterium lividum) |
The results of the alignment to the best BLASTX hits, their identity (%), and accession numbers in the nonredundant GenBank database are provided. MIC, minimal inhibitory concentration.