Fig. 6.

Antioxidant application to aged WT skeletal muscle reduces age-associated SR Ca²⁺ leak. (A) Representative immunoblot of immunoprecipitated RyR1 from aged murine skeletal muscle. For DTT treatment, SR vesicles were preincubated with 1 mM DTT. (B) Bar graphs showing quantification of the immunoblots in A. (C) Bar graph representing Ca²⁺ leak in SR microsomes of skeletal muscles from aged WT mice. For N₂ treatment, solutions was prebubbled with 100% N₂ for 1 h. (D) Bar graph representing average Ca²⁺ spark frequency in permeabilized FDB muscle fibers from aged WT mice. Data are mean ± SEM (n = 19–22 cells from three mice per group; *P < 0.05 vs. aged WT; **P < 0.01 vs. aged WT, ANOVA).