Figure 3. Immunofluorescent microscopy of YFP and F4/80 costaining in the cortex of YARG mice post-TBI.

Representative images from colabeling experiments of YFP, F4/80, and DAPI on the contralateral (1^st column) and ipsilateral cortex (2^nd column) of brain tissue four days post-TBI. Scale bar represents 50 μm. The top areas of columns 1 and 2, devoid of DAPI staining, are outside the boundary of brain tissue. The third column shows higher magnification of macrophages in the ipsilateral cortex; scale bar 25 μm. Arrows indicate cells colabeled with anti-YFP and anti-F4/80 antibodies. Data shown are from one experiment performed and are representative of four experiments performed analyzing three animals, 10 sections per animal.