Figure 5. Differences in transcriptional responses between Arg1⁺ and Arg1^- brain wound macrophages selecting for genes associated with M1 and M2 polarization and/or with other selected macrophage functions genes.

(A) Gene expression analysis comparing Arg1⁺ TBI macrophages and Arg1^- TBI macrophages one day after injury. Thirty-five selected genes of interest with significant relative differences of close to two-fold or higher are shown. Each column represents a separate experiment. Gene expression was log₂ transformed and median-centered across genes. Yellow represents a relative increase in expression and blue signifies a relative decrease. Expressed genes are grouped by function. (B) Average gene expression differences from the data in Figure 4A were quantified and ratios of gene expression levels comparing Arg1⁺ brain wound macrophages to Arg1^- brain wound macrophages are shown.