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. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: Methods Mol Biol. 2013;931:1–27. doi: 10.1007/978-1-62703-056-4_1

Fig. 6.

Fig. 6

(a) Six by six pixel array (see Fig. 5a). Pixels have discrete intensity values, positional information within the array, and may imply a three-dimensional voxel (volume element). (b) The same array as (a) after a moderately aggressive brightness and contrast adjustment using ImageJ 1.43 m (115). (c) The same array as (a) after applying the Adobe Photoshop® CS3 sharpen filter. Graph: (key: light grey = (a), medium grey = (b), black = (c)). The graph shows the intensity values of the fourth row of pixels (arrow) in the images. The original image contains values that fit within the range of 0–255 (black-white). After the brightness and contrast adjustment, or the sharpening filter, a number of the intensity values have been truncated (asterisk) since they exceeded the 0–255 range, leaving just the maximum or minimum values. The relationship of these truncated values to those of the neighboring pixels has been lost (see Subheadings 6.1 and 6.5).