Table 1.
Spot intensity and HIV-1 Western Blot bands for Multispot HIV-2 and HIV-1 and HIV-2 reactive specimens. The spot intensity was defined as follows: no spot visible (−); light purple spot color (+/−); and clearly defined purple spot color (+).
| Samples number | Screening assay | Supplemental confirmatory test
|
WB bands | Infection status | |||
|---|---|---|---|---|---|---|---|
| Multispot
|
Immunoblot | Western Blot | |||||
| HIV-1 spots | HIV-2 spot | HIV-2 | HIV-1 | ||||
| 1 | 3rd Gen | − | + | Pos | Ind | 55, 40, 31, 24 | HIV-2 |
| 2 | 3rd Gen | − | + | Pos | Pos | 160, 55, 31, 24 | HIV-2 |
| 3 | 3rd Gen | − | + | Pos | Pos | 160, 31, 24 | HIV-2 |
| 4 | 3rd Gen | + | +/− | Neg | Pos | All | HIV-1 |
| 5 | 4th Gen | + | + | Pos | Pos | 160, 120, 31, 24 | HIV-2 |
| 6 | 4th Gen | + | +/− | Neg | Pos | All | HIV-1 |
| 7 | 4th Gen | + | +/− | Neg | Pos | All | HIV-1 |
| 8 | 4th Gen | +/− | +/− | Neg | Ind | 160 (+/−) | HIV-1 NC |
HIV-1 NC: inadequate specimen for HIV RNA confirmation; 3rd Gen: 3rd generation assay; 4th Gen: 4th generation assay; Ind: indeterminate; Pos: positive; Neg: negative.