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. Author manuscript; available in PMC: 2014 Oct 27.
Published in final edited form as: J Biomol Screen. 2012 Nov 27;18(4):498–503. doi: 10.1177/1087057112468765

Figure 2.

Figure 2

On-line superoxide detection by 10 μM DHE in xanthine oxidase system at 405 nm excitation and 570 nm emission. Freshly prepared solutions of 0.1 mU/ml xanthine oxidase and DHE from DMSO stock were mixed in 50 mM PSB pH 7.4. Superoxide production was initiated by 0.2 mM xanthine added from saline stock and inhibited by 50 units/ml SOD. Inset shows typical HPLC analysis of 2OH-E accumulation in samples containing: DHE, xanthine oxidase plus xanthine (DHE/XO/X); DHE, xanthine (DHE/X); DHE and xanthine oxidase (DHE/XO) or DHE. There were no differences in HPLC chromatograms after 10 min.