Figure 6.

ISP2 is necessary for sustained nuclear NF-κB in macrophages infected with L. major. A, C) Cells were infected for 2, 5, or 8 h, and the nuclear extracts were processed for Western blot analysis with antibodies to NF-κB subunit p65 (A), IRF3 (C), or nuclear lamin, as the loading control. B) NF-κB promoter activation. RAW cells were transiently transfected with the reporter plasmid p6Kb-LUC, which contains 6 consensus-binding sites for NF-κB, located upstream of the luciferase gene, and were infected for 24 h. Noninfected cells were used as negative controls for basal luciferase activity. Experiments were performed 2 independent times. Open bars: WT; solid bars: Δisp2/isp3; gray bars: Δisp2/isp3:ISP2-ISP3. *P < 0.05.