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. 2014 Oct 2;15(10):481. doi: 10.1186/s13059-014-0481-4

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© Bayne et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sde2, Smd3, Saf1 and Saf5 affect RNAi-dependent heterochromatin formation. (A) cen1:ade6 ⁺ silencing assay. Equivalent cell numbers of the indicated strains were spotted in serial dilutions on non-selective plates (N/S), or plates containing 10 ug/mL adenine (LOW ADE) or no adenine (-ADE). (B) ChIP analysis of H3K9me2 levels at the centromeric outer repeats (cen-dg) relative to the act1 ⁺ gene, normalised to wild-type. (C) Northern analysis of centromeric siRNAs. snoRNA58 (snR58) is a loading control.