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. 2014 Oct 2;15(10):481. doi: 10.1186/s13059-014-0481-4

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© Bayne et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Csn1 and Csn2 contribute to heterochromatin integrity via regulation of Epe1. (A, B) Analysis of genetic interactions of csn1Δ and csn2Δ with ddb1Δ. (A) Assays for silencing of reporter genes at the centromere (cen1:ade6 ⁺ ) and mating-type locus (mat3-M:ura4 ⁺ ). Plates are limiting adenine (LOW ADE), non-selective (N/S), lacking uracil (-URA) or supplemented with FOA (+FOA). (B) qRT-PCR analysis of centromeric outer repeat (cen-dg) and mating-type locus (mat) transcript levels relative to act1 ⁺, normalised to wild-type. (C) ChIP analysis of Epe1-FLAG levels at centromeric outer repeats (cen-dg) relative to act1 ⁺, normalised to wild-type. (D) Assay for silencing at the mating-type locus (mat3-M:ura4 ⁺).