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A representation of the experimental design; each biological replicate comprising equal quantities of RNA from six individuals
| Sac fry | Feeding fry | |
|---|---|---|
| Wild; F ♀ × F ♂ | 6 pools | 6 pools |
| Hybrid; M ♀ × F ♂ | 6 pools | 6 pools |
| Domesticated; M ♀ × M ♂ | 6 pools | 6 pools |
