Table 3.
A comparison of gene expression ratios of domesticated and hybrid salmon with respect to wild individuals evaluated using RT-qPCR and microarray analysis
| Sac fry | Feeding fry | |||||||
|---|---|---|---|---|---|---|---|---|
| Domesticated | Hybrid | Domesticated | Hybrid | |||||
| Target | RT-qPCR | MA | RT-qPCR | MA | RT-qPCR | MA | RT-qPCR | MA |
| MHCII | -1.48 | -1.37 | (-1.17) | (-1.10) | -1.95 | -2.09 | -1.24 | -1.38 |
| EPHX | 1.27 | 2.24 | 1.20 | 1.57 | 1.23 | 2.08 | 1.20 | 1.55 |
| IGF | -1.11 | (1.39) | (1.01) | (1.56) | (1.08) | (-1.14) | (1.05) | (1.79) |
| Pesc | (1.02) | 2.82 | (1.03) | 1.91 | -1.15 | 2.43 | -1.10 | (1.36) |
| Poly10 | -2.31 | -6.72 | -1.28 | (-1.78) | -1.61 | -3.19 | -1.28 | -1.63 |
Microarray values are based on T-tests (unpaired unequal variance, p ≤ 0.01 and FC > =1.3), whereas RT-qPCR ratios were obtained by REST2009 (p ≤ 0.05). Non-significant values are shown in parenthesis. Ratios lower than 1 are expressed as -1/ratio to obtain an equivalent value to ratios above 1.