Fig. 4.
Agrin 000 and agrin 748 reduce the proliferation rate of bEnd5 cells. a Cellular proliferation was measured by 3H-thymidine uptake after 48 h in culture (CPM counts per minute). The values of each experiment were normalized to the respective control condition. Bars represent the mean of six independent experiments (± SEM). *P < 0.05. b bEnd5 cells were counted after 48 h in culture in the presence of agrin 000, agrin 748, or under control conditions. Symbols represent the triplicates of one experiment, and the mean (horizontal line) is shown for better visualization. c After 48 h, the percentage of apoptotic and necrotic cells was analyzed by Annexin V (Ann) and propidium iodide (PI) staining. Positive bEnd5 cells were analyzed by flow cytometry. Symbols represent values of two independent experiments, and the horizontal lines represent the means