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. 2014 Oct 17;12(10):5222–5239. doi: 10.3390/md12105222

Figure 1.

Figure 1

RTCA-generated time-dependent cell response profiles after treatment with ascidian extracts. (A–F) The real-time profiles display the seeding period (−24–0 h) followed by the treatment period (0–72 h) of MDA-MB-231 breast cancer cells. At 0 h, the indicated ascidian extracts were added at a final concentration of 1 µge/µL, and the cell index (CI) was measured every 2 h. As controls, cells were treated with DMSO (0.1%), doxorubicin (Dox, 10 µM), or remained untreated (medium); (G) Representative RTCA profiles of non-malignant NFF cells treated with the indicated ascidian extracts and controls.