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. 2014 Oct 23;12(10):5295–5315. doi: 10.3390/md12105295

Figure 6.

Figure 6

Inhibition of p38 and JNK activities rescued the cell cytotoxicity of AGS cells caused by 13-AC. (A) Two inhibitors, SB203580 (p38-specific inhibitor) and SP600125 (JNK-specific inhibitor), were added to AGS cells treated with 13-AC and the cell viability was detected by MTT assay as described above. An increased cell viability of the 13-AC-treated AGS cells was observed following addition of SB203580 and SP600125, but not PD98059. The results shown are representative of three independent experiments (# p < 0.05 as compared with the control). (B) Validation of the expression levels of mitochondrial-related proteins affected by two inhibitors. β-actin was used as the protein loading control. (C) Decrease in the apoptotic AGS cells characterized by TUNEL and DAPI staining assays. TUNEL and DAPI staining showed decreases in the 13-AC-induced apoptotic characteristics when treated with SB203580 and SP600125. Scale bars = 50 μm.