Table 1.
Pyrosequencing assay information.
| Gene | Chromosome | CpG Position * | Primers: Forward (F), Reverse (R), and Sequencing (S) |
|---|---|---|---|
| CD14 | 5 | 140632047 | F: GGGTTTATAGAGGAGGGAATTGA |
| 140632043 | R: Biotin-AAACCCCATCCAACCCCTAT | ||
| 140632041 | S: TGTAGGGTTTTGGGG | ||
| 140632031 | |||
| iNOS | 17 | 27799241 | F: TTAGGGTTAGGTAAAGGTATTTTTGTTT |
| 27799233 | R: Biotin-CAATTCTATAAAACCACCTAATAATCTTAA | ||
| S: TAAAGGTATTTTTGTTTTAA | |||
| Et-1 | 6 | 12290364 | |
| 12290366 | F: TTGTTTGGGGTTGGAATAAAGT | ||
| 12290380 | R: Biotin-ATCCTTCAACCCAAATACCCTTTT | ||
| 12290387 | S: GGTAGAGAGTTGTTTAAGTT | ||
| 12290391 | |||
| HERV-w | 7 | 92468887 | F: AAGTTATAGTTGAAGGAAGA |
| 92468872 | R: Biotin-CAATCCCCCATCTCAACAA | ||
| 92468856 | S: AGTTTAAGATTAAGATTTAT | ||
| TNFα | 6 | 2874672 | F: Biotin-TGAGGGGTATTTTTGATGTTTGT |
| 2874678 | R: TTAATAATTATTTTTATATATTTT | ||
| 2874680 | S: ATAAATTTTATATTTTTTAT | ||
| 2874695 |
* According to the University of California, Santa Cruz, UCSC Genome Browser, December, 2013, release (GRch38/hg38) [23].