YM155 induced cell death and autophagy in ACC xenograft. a Immunohistochemical analysis of the survivin, cyclin D1, cleaved caspase 3(CL-Casp3) and Beclin1in both vehicle and YM155-treated ACC-2 xenograft tissues, in situ apoptosis were stained by TUNEL assay. b The expression of survivin, LC3II/LC3I, SQSTM1, cleaved PARP (CLPARP) and cleaved caspase 3(CL-Casp3) by western blot. c Relative quantitative data were calculated by ImageJ. *P < 0.05; **P < 0.01 as compared with the control group. Student t test was used