White blood cell focusing behavior as a function of flow rate Q and RBC volume fraction fRBC. For fRBC = 0, values of Q correspond to Rp = 0.27, 0.80, and 2.41. For fRBC = 0.07, values of Q correspond to Rp = 0.22, 0.66, and 1.99. For fRBC = 0.33, values of Q correspond to Rp = 0.14, 0.40, and 1.21. (a) The in-focus vertical position yf and in-focus lateral distance zf from the channel centerline for white blood cells were used to construct a cross-sectional particle histogram. (b) The dependence of particle diameter a on in-focus lateral distance zf can be illustrated using a particle scatter plot. The dotted line represents the location of the sidewall given a non-deformable microchannel.