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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Mol Med (Berl). 2014 Jan 25;92(5):473–485. doi: 10.1007/s00109-014-1120-y

Figure 8. ASM overexpression increases autophagolysosomes (APLs) formation in CASMCs upon atherogenic stimulation.

Figure 8

(A) Effects of 7-Ket (10 μM for 0.5, 6 or 24 hours) or amitriptyline (Ami; 10 μM for 24 hours) on ASM activity in Smpd1+/+ CASMCs. (B) Mouse CASMCs were transfected with ASM cDNA plasmid encoding Smpd1 gene or control vector with scrambled cDNA (Scr) by Nucleofection technology. Summarized data showing ASM activity in Smpd1+/+ and Smpd1−/− CASMCs transfected with ASM cDNA plasmid or scramble. (C) Summarized red-to-green fluorescence ratio of acridine orange staining in ASM cDNA or scramble transfected Smpd1+/+ or Smpd1−/− CASMCs under control condition or with atherogenic stimulation by 7-Ket (10 μM; 24 hours). *P<0.05 vs. Smpd1+/+ control; #P < 0.05 Smpd1−/− vs. Smpd1+/+; $P < 0.05 vs. Scr+7-Ket (n=4).