Fig. 5.

Effect of 25(OH)D₃ and VDR siRNA on p38 phosphorylation in LPS-activated BV2 cells. BV-2 cells were treated with 25(OD)D₃ in the presence or absence of VDR siRNA (50 µmol/L). Treatment with 100 µmol/L 25(OH)D₃ inhibited the increased phosphorylation of p38 in 100 ng/ml LPS-activated BV2 cells. The extent of phosphorylation of p38 was measured by the ratio of phospho p38 to p38. VDR siRNA blocked the inhibitory effect of 25(OH)D₃ on the LPS-induced phosphorylation of p38. The inset shows the representative Western blotting product of p38 and phospho-p38 (p-p38). Mean values were obtained from three independent experiments. ^*p<0.05 when compared to the untreated control group (Student's t-test).