Figure 6.

Soft X-ray tomography of mouse olfactory sensory neurons (OSNs). (a)–(c) Control OSN; (d)–(f) OSN expressing exogenous Lamin B receptor (LBR). (a, d) Orthoslices from the tomographic reconstructions; (b, e) three orthogonal orthoslices through the same nuclei shown in (a, d), respectively; (c, f) surface views of the same nuclei revealing the highly folded nuclear envelope in the enlarged nucleus expressing LBR (f). Asterisks show the mass of pericentromeric heterochromatin located in the center of the nucleus in control OSN (a, b) and displaced in the nucleus expressing LBR (d, e); arrows in (a, d) point to the nuclear periphery, which appears thicker in (d) after expression of LBR. Reproduced from Clowney et al. (2012 ▶). Scale bar = 2 µm.