Table 4.

Effects of RHP on catabolic gene expression in human primary chondrocytes. IL-1β-stimulated cells were cultured with 125–500 μg/mL RHP for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Values for (IL-1β stimulated) cells and (500 μg/mL RHP + IL-1β) stimulated cells are given.

Gene	IL-1β	IL-β + RH-A (500 μg/mL)		IL-1β + RH-B (500 μg/mL)		P value RH-A versus RH-B
	Fold change	Fold change	P valuea	Fold change	P value
MMP-1	28	16	0.001	26	0.620	0.032
MMP-3	286	120	0.001	160	0.003	NSb
MMP-13	1.7	0.2	<0.001	0.5	<0.001	0.040
ADAMTS-4	10	5	0.006	8	0.295	NS
CXCL2/MIP-2	467	122	<0.001	180	<0.001	0.091
CXCL20/MIP-3α	642	253	0.001	331	0.010	NS
CCL5/RANTES	176	127	0.008	151	0.241	NS
CXCL8/IL-8	797	214	<0.001	301	<0.001	0.087
IL-1α	466	200	0.001	292	0.032	NS
IL-6	1454	897	0.022	746	0.006	NS
TNF-α	1120	1081	0.727	1704	0.005	NS

^a P values: significance value between (IL-1β) and (IL-1β + substance) treatment.

^bNot significant.