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. 2014 Oct 13;2014:519528. doi: 10.1155/2014/519528

Figure 4.

Figure 4

CVB3 copy numbers of the viral RNA genome (+) strand as well as the intermediate (−) RNA strand for virus replication. The copy number of the viral RNA genome (+) strand and the copy number of the intermediate (−) strand needed for virus replication were determined using strand-specific primers for reverse transcription followed by TaqMan analysis. (a) Copy numbers of (+) strand (viral RNA genome) of CVB3 6 hours and 24 hours after infection of murine cardiac fibroblasts, murine cardiomyocytes (HL-1 cell line), and murine macrophages (RAW cell line). (b) Copy numbers of (−) strand (intermediate RNA strand for virus replication) of CVB3 6 hours and 24 hours after infection of murine cardiac fibroblasts, murine cardiomyocytes (HL-1 cell line), and murine macrophages (RAW cell line). ((c), (d)) Ratio between copy numbers 24 hours compared to copy numbers 6 hours after CVB3 infection for (c) (+) strand and (d) (−) strand. Ratios higher than one demonstrate virus replication. ((a), (b))  *Significantly different versus copy numbers 6 hours after infection; *P < 0.05; **P < 0.01. Data are plotted as mean ± SEM. ((c), (d))  *Significantly different versus cardiac fibroblasts; ***P < 0.001 §significantly different versus cardiomyocytes; §§§ P < 0.001. Ratios are plotted as box plots with median.