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. 2014 Jun 13;21(11):1696–1708. doi: 10.1038/cdd.2014.75

Figure 3.

Figure 3

Genetic invalidation of Glra2 disrupts cortical progenitor homeostasis. (a, b) Immunolabelings showing Pax6-expressing APs (red), Tbr2-expreessing BPs (green) and progenitors undergoing mitosis that are positive for phospho-histone H3 (pHH3, blue) in brain sections from WT (a) or Glra2 KO (b) E13 embryos. (c–f) Histograms representing numbers of Pax6-postive APs per field (c), Tbr2-positive BPs per field (d), percentage of APs undergoing mitosis per field (Pax6- and pHH3-positives, (e) and percentage of BPs undergoing mitosis per field (Tbr2- and pHH3-positives, f). Values are expressed as mean±S.E.M., n=14 and 16 brain sections taken from four brains per genotype; **P<0.01, ***P<0.001, t-test (a–f). (g–l) Immunolabelings showing Pax6-expressing APs (red), Tbr2-expressing BPs (green) and progenitors undergoing mitosis that are positive for phospho-histone H3 (pHH3, blue) in brain sections from WT (g) or Glra2KO (h) E15 embryos. (i–l) Histograms representing numbers of Pax6-postive APs per field (i), Tbr2-positive BPs per field (j), percentage of APs undergoing mitosis per field (Pax6- and pHH3-positives, (k) and percentage BPs undergoing mitosis per field (Tbr2- and pHH3-positives, l). Values are expressed as mean±S.E.M., n=16 brain sections taken from four brains per genotype; *P<0.05, **P<0.01, ***P<0.001, T-test. (g–l). Scale bar, 50 μm (b). Arrows show pHH3-expressing cells (a, b, g, h)