Figure 4.

Genetic inactivation of Glra2 disrupts mitotic cleavage plane division. (a, b) Immunolabelings showing cells expressing phospho-histone H3 (pHH3, purple) and undergoing mitosis in the cortical wall of from WT (a) or Glra2 KO (b) E13 embryos. (c, d) Histograms representing absolute numbers of APs undergoing mitosis per field (Pax6- and pHH3-positives, (c) and absolute number of BPs undergoing mitosis per field (Tbr2- and pHH3-positives, d). Values are expressed as mean±S.E.M., n=14 and 16 brain sections taken from four brains per genotype; *P<0.05, t-test (c, d). (e–h) Scheme representing mitotic cleavage plane angle for APs (e), representative mitotic cleavage plane angles for APs undergoing mitosis in WT (f) or Glra2 KO E13 embryos. Distribution of mitotic cleavage plane angles of WT or Glra2 KO APs; **P<0.001, Mann–Whitney test (h)