Figure 5.

Genetic targeting of Glra2 impairs apico-basal transition in the developing cortex. (a, b) In utero electroporation of GFP-expressing plasmids was conducted on WT (a) or Glra2 KO (b) E13 embryos to target apical progenitors that lie against the ventricular membrane. Immunolabelings were performed 36 h later on brain sections from electroporated brain and show cells expressing Tbr2 (purple), GFP (green) and the nuclear label Dapi (blue). Scheme of the in utero electroporation procedure (c), see also material and methods). Histogram representing the percentage of electroporated cells (GFP+) expressing or lacking Tbr2 in the ventricular zone (VZ), subventricular zone (SVZ) or intermediate zone and cortical plate (IZ and CP, respectively) (d); mean±S.E.M., n=6 and 8 brain sections taken from different brains per genotype; * P<0.05, t-test)