Skip to main content
. 2014 Jun 13;21(11):1696–1708. doi: 10.1038/cdd.2014.75

Figure 6.

Figure 6

Genetic targeting of Glra2 impairs cell cycle exit and neuronal differentiation in the developing cortex. (a, b), Immunolabelings performed on brain section showing cells expressing BrdU (red), Ki67 (green) and the nuclear label Dapi (blue) in WT (a) or Glra2-KO (b) E13 embryos. Percentage of cells that exit the cell cycle per field (c; BrdU+/Ki67- cells; mean±S.E.M., n=11 and 15 brain sections taken from three and four brains for WT and Glra2 KO embryos, respectively). (d, e) Immunolabelings performed on brain section showing cells expressing Tbr1 (red), Tbr2 (green) and the nuclear label Dapi (blue) in WT (d) or Glra2 KO (e) E12 embryos. (f–h), histograms representing number of cells co-labeled for Tbr1 and Tbr2, per field (f), the number of cells expressing Tbr1 per field (g) and the number of cell expressing Tbr2 per field (h). Values are expressed as mean±S.E.M., n=16 brain sections taken from four brains per genotype (f–h); *P<0.05, **P<0.01, t-test