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. 2014 Jun 13;21(11):1696–1708. doi: 10.1038/cdd.2014.75

Figure 7.

Figure 7

Genetic targeting of Glra2 results in a reduced number of projection neurons in upper and deep cortical layers. (a–d) mRNA labeling after in situ hybridization in brain sections from P0 WT or Glra2 KO mice. Latin numbers indicate cortical layer identity. (e-i) Immunolabelings performed on brain section showing cells expressing Cux1 (red, (e), Ctip2 (red, (f) Tbr1 (green, (f) and the nuclear label Dapi (blue) in P0 WT or Glra2 KO mice. (g-i) Histograms representing the number of Cux1-expressing neurons per field (g), the number of Ctip2-expressing neurons per field (h) and the number of Tbr1–expressing neurons per field (i); mean±S.E.M., n=10 brain sections taken from three brains per genotype; * P<0.05, t-test). (g–i) Values are expressed as mean±S.E.M., n=11 brain sections taken from three brains per genotype; * P<0.05, t-test