Figure 6.

Prkar1a deletion did not promote carcinogen-induced tumorigenesis in mice. (a) Kaplan–Meier curve for tumour-free survival after the administration of DMBA and TPA. (b) Western blot analysis of skin tissues 5 days post administration of tamoxifen to determine the levels of BIM and PUMA. In each western blot, spleen extracts from wild-type, Bim^−/− or Puma^−/− mice were used to demonstrate antibody specificity. (c) RNA copy measurement from skin samples 5 days post tamoxifen treatment by digital droplet PCR. Error bars±S.E.M., n=3