Table 1.
Bacterial strains and plasmids used in this work.
| Bacterial strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Escherichia coli | ||
| DH5α λpir | Cloning host; F−λ−endA1 glnX44(AS) thiE1 recA1 relA1 spoT1 gyrA96(NalR) rfbC1 deoR nupG Φ80(lacZΔM15) Δ(argF-lac)U169 hsdR17(rK−mK+), λpir lysogen | Hanahan and Meselson (1983) |
| CC118 λpir | Cloning host; Δ(ara-leu) araD ΔlacX174 galE galK phoA thiE1 rpsE rpoB(RifR) argE(Am) recA1, λpir lysogen | Herrero et al. (1990) |
| S17-1 λpir | Cloning host; F−recA1 endA1 thiE1 pro-82 creC510 hsdR17 RP4-2(Km:Tn7 Tc:Mu-1), λpir lysogen, SmR | de Lorenzo et al. (1993) |
| S17P | Same as S17-1 λpir but carrying plasmid pBAMD1-6-pha (phaC1AB1+), SmR ApR GmR | This work |
| HB101 | Helper strain; F−λ−hsdS20(rB−mB−) recA13 leuB6(Am) araC14 Δ(gpt-proA)62 lacY1 galK2(Oc) xyl-5 mtl-1 thiE1 rpsL20(SmR) glnX44(AS) | Boyer and Roulland-Dussoix (1969) |
| BW25113 | Wild-type strain; F−λ−Δ(araD-araB)567 ΔlacZ4787(:rrnB-3) rph-1 Δ(rhaD-rhaB)568 hsdR514 | Datsenko and Wanner (2000) |
| JW2293-1b | Same as BW25113 but Δpta779:aphA, KmR | Baba et al. (2006) |
| JW2293P | Same as JW2293-1 but carrying plasmid pAeT41 (phaC1AB1+), ApR | This work |
| TA2293P | Same as JW2293-1 but ykgH:mini-Tn5(phaC1AB1), expresses the pha gene cluster from Cupriavidus necator as a chromosomal integration, KmR GmR | This work |
| Pseudomonas putida | ||
| KT2440 | Wild-type strain; mt-2 derivative cured of the TOL plasmid pWW0 | Bagdasarian et al. (1981) |
| Plasmids | ||
| pRK600 | Helper plasmid used for conjugation; ori(ColE1), RK2(mob+ tra+); CmR | Kessler et al. (1992) |
| pBAM1 | Mini-Tn5 delivery plasmid; ori(R6K), standard multiple cloning site; ApR KmR | Martínez-García et al. (2011) |
| pSEVA111 | Cloning vector; ori(R6K), standard multiple cloning site; ApR | Silva-Rocha et al. (2013) |
| p-R-SETA111 | Cloning vector; ori(R6K), standard multiple cloning site, tnpA; ApR | This work |
| pBAMD1-2 | Mini-Tn5 delivery plasmid; ori(R6K); ApR KmR | This work |
| pBAMD1-4 | Mini-Tn5 delivery plasmid; ori(R6K); ApR SmR/SpR | This work |
| pBAMD1-6 | Mini-Tn5 delivery plasmid; ori(R6K); ApR GmR | This work |
| pAeT41 | Derivative of cloning vector pUC18 (Thermo Fisher Scientific Inc.; Pittsburgh, PA, USA) bearing a ca. 5-kb SmaI/EcoRI DNA fragment spanning the phaC1AB1 gene cluster from C. necator; ApR | Peoples and Sinskey (1989) |
| pBAMD1-6-pha | Derivative of pBAMD1-6; delivery plasmid used to insert the phaC1AB1 gene cluster from C. necator into the chromosome of recipient E. coli strains; ApR GmR | This work |
aAntibiotic markers: Ap, ampicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Nal, nalidixic acid; Rif, rifampicin; Sm, streptomycin; Sp, spectinomycin; and Tc, tetracycline.
bStrain obtained from the E. coli Genetic Stock Center (Yale University, New Haven, CT, USA).