Table 2.
Oligonucleotides used in this work to identify and sequence the chromosomal locus of mini-Tn5 insertions.
| Oligonucleotide | Sequence (5′ → 3′) | Use and reference |
|---|---|---|
| ARB6 | GGCACGCGTCGACTAGTACNNNNNNNNNNACGCC | Arbitrary PCR, round 1 (Pratt and Kolter, 1998) |
| ARB2 | GGCACGCGTCGACTAGTAC | Arbitrary PCR, round 2 (Pratt and Kolter, 1998) |
| pBAM-ME-I-Ext-R | CTCGTTTCACGCTGAATATGGCTC | Arbitrary PCR for pBAMD1-2, round 1 (Martínez-García et al., 2011) |
| pBAM-ME-I-Int-R | CAGTTTTATTGTTCATGATGATATA | Arbitrary PCR for pBAMD1-2, round 2, and sequencing (Martínez-García et al., 2011) |
| ME-O-Km-Ext-F | CGTCTGTTTCAGAAATATGGCAT | Arbitrary PCR for pBAMD1-2, round 1 |
| ME-O-Km-Int-F | ATCTGATGCTGGATGAATTTTTC | Arbitrary PCR for pBAMD1-2, round 2, and sequencing |
| ME-I-Sm-Ext-R | ATGACGCCAACTACCTCTGATA | Arbitrary PCR for pBAMD1-4, round 1 |
| ME-I-Sm-Int-R | TCACCGCTTCCCTCATGATGTT | Arbitrary PCR for pBAMD1-4, round 2, and sequencing |
| ME-O-Sm-Ext-F | CTTGGCCTCGCGCGCAGATCAG | Arbitrary PCR for pBAMD1-4, round 1 |
| ME-O-Sm-Int-F | CACCAAGGTAGTCGGCAAAT | Arbitrary PCR for pBAMD1-4, round 2, and sequencing |
| ME-O-Gm-Ext-F | GCACTTTGATATCGACCCAAGT | Arbitrary PCR for pBAMD1-6, round 1 |
| ME-O-Gm-Int-F | TCCCGGCCGCGGAGTTGTTCGG | Arbitrary PCR for pBAMD1-6, round 2, and sequencing |
| ME-I-Gm-Ext-R | GTTCTGGACCAGTTGCGTGAG | Arbitrary PCR for pBAMD1-6, round 1 |
| ME-I-Gm-Int-R | GAACCGAACAGGCTTATGTCA | Arbitrary PCR for pBAMD1-6, round 2, and sequencing |