Table 3.
Insertion sites of the mini-transposon born by the different pBAMD1-x vectors in the genome of P. putida KT2440a.
| Clone | End | Genome coordinate (bp) | PP number | Strand | Gene name and putative function |
|---|---|---|---|---|---|
| KT-K1 | ME-I | 5,825,403 | PP5103 | − | trmB, tRNA (guanine-N7-) methyltransferase |
| KT-K2 | ME-I | 425,161 | PP0349 | − | PepSY-associated transmembrane helix domain-containing protein |
| KT-K3 | ME-I | 5,272,887 | PP4648 | + | rRNA (guanine-N2-) methyltransferase |
| KT-K5 | ME-O | 864,889 | PP0745 | − | uraA, uracil-xanthine permease |
| KT-K6 | ME-O | 4,661,528 | PP4124 | + | nuoG, NADH dehydrogenase subunit G |
| KT-K7 | ME-O | 1,261,995 | PP1104 | − | Succinyl-glutamate desuccinylase/aspartoacylase |
| KT-K8 | ME-O | 727,311 | PP0621 | − | Hypothetical protein |
| KT-G1b | ME-O | 2,988,216 | PP2612 | − | Hypothetical protein |
| 4,108,354 | PP3616 | + | Hypothetical protein | ||
| KT-G2 | ME-O | 1,313,801 | PP1145 | − | hepA, ATP-dependent helicase |
| KT-G3 | ME-O | 3,002,652 | PP2626 | + | Hypothetical protein |
| KT-G4 | ME-O | 3,251,143 | PP2847 | + | ureJ, hydrogenase/urease accessory protein |
| KT-G6 | ME-I | 4,444,716 | PP3941 | − | Isochorismatase superfamily hydrolase |
| KT-G7 | ME-I | 4,792,981 | PP4221 | − | Non-ribosomal peptide synthetase |
| KT-G8 | ME-I | 1,959,862 | PP1757 | + | bolA, BolA family protein |
| KT-S1 | ME-I | 4,463,594 | PP3956 | + | Hypothetical protein |
| KT-S2 | ME-I | 3,552,337 | PP3136 | NA | Intergenic region (PP3136 and PP3137) |
| KT-S3 | ME-I | 4,671,241 | PP4132 | − | Hypothetical protein |
| KT-S4 | ME-I | 35,484 | PP0031 | − | Hypothetical protein |
| KT-S5 | ME-O | 2,422,455 | PP2123 | + | moeA, molybdopterin biosynthesis enzyme |
| KT-S6 | ME-O | 6,021,399 | PP5272 | − | Hypothetical protein |
| KT-S7 | ME-O | 381,423 | PP0317 | − | Methyl-accepting chemotaxis sensory transducer |
| KT-S8 | ME-O | 328,477 | PP0270 | − | Integral membrane sensor signal transduction histidine kinase |
| KT-K2-G1 | ME-O | 4,892,623 | PP4301 | + | pykF, pyruvate kinase |
| KT-K2-G2 | ME-O | 3,749,626 | PP3313 | − | Heat shock protein |
| KT-K2-G3 | ME-O | 5,842,545 | PP5120 | + | Conifer aldehyde dehydrogenase |
| KT-K2-G4 | ME-O | 1,373,720 | PP1199 | − | Hypothetical protein |
| KT-K2-S1 | ME-O | 2,904,080 | PP2556 | − | Chromate transporter |
| KT-K2-S2 | ME-O | 5,347,381 | PP4703 | + | Hypothetical protein |
| KT-K2-S3 | ME-O | 1,542,387 | PP1353 | + | Mechanosensitive ion channel protein MscS |
| KT-K2-S4 | ME-O | 5,892,275 | PP5166 | − | Fis family transcriptional regulator |
| KT-K2-G1-S1 | ME-O | 3,635,742 | PP3202 | + | Predicted exporter of the RND superfamily |
| KT-K2-S4-G1b | ME-O | NA | PP0168 | + | lapA, surface adhesion protein |
aThe insertion sites were ascertained by means of arbitrary PCR with the oligonucleotides indicated in Table 2, and the assigned function of the corresponding ORF is given according to the information available in the Pseudomonas Genome Database (Winsor et al., 2011). NA, not available.
bIn the two transconjugant clones indicated, the insertion site of the mini-Tn5 element could not be unambiguously identified.