Figure 4.

Signal pathway activation in sponge granuloma sections from ApoE KO or ApoE/Rag-1 DKO mice. Sections from ApoE KO (A,C,E) or DKO (B,D,F) mice were stained to show immunolocalization of NF-κB (A,B) or phosphorylated STAT1 (C,D). Control sections (E,F) were exposed to non-immune IgG. Positive staining appears brown (DAB), nuclei blue-purple (hematoxylin), and sponge spicules light blue. Sections from either mouse strain contained many cells with positive cytoplasmic staining (arrowheads). Some cells did not contain detectable levels of NF-κB or phospho-STAT-1, while others appeared to have NF-κB or phospho-STAT-1 present in their nucleus (arrows) (Magnification: ×600).