Table 1.
Summary characterization and comparison of plasmid backbones and accessory genes.
| Genetic backbone | Accessory components | |||||||
|---|---|---|---|---|---|---|---|---|
| Replication | Mobility | Maintenance | Resistance genes | |||||
| Plasmid (source) | Inc group | Tra genes (mob genes) | Trb genes | Plasmid addiction (TA systems) | †Regulatory and stability | Integron | Antibiotic | Heavy metal |
| pTOR_01 20,914 bp (influent) | repB/IncU | N | None | None predicted | parA/B | None | mph(A), mrx(A), mphR(A) | mpr |
| pRWC72a 61,919 bp (influent) | trfA/IncP-1β | P-T4SS; C,D,E,FGI,J,K,L,M | A,B,C,D,E,F,G,H,I,J,K,L,M,N,OP | yacA, yacB | ssb,parA/B, krfA/B,incC1, korB/C,korA, kleA/B/C/E, klcA, krfA | ΔintI1, class1; qacEΔ1, sulI, orf5 | mph(B), mrx(B), mphR(B), tetA, tetR | None |
| pTOR_02 28,080 bp (effluent) | col-E related | mobA | None | None predicted | None predicted | intI1, class1; dhfrA, aadA2, qacEΔ1, sulI, orf5 | mph(A), mrx(A), mphR(A) | merA, merD, merE, merP, merT, merR |
| pEFC36a 87,419 bp (effluent) | repA1/IncFII | F-T4SS; B,C,D,F,G,H,IJ,K,L,M,N,S,T,U,V,W, X | A,B,C,F, I, J, O | type I TA system: hok/sok | conjugation: finO, finP, traJ, traM, traY; partition: stbA/B (parM/R) | intI1, class1; dhfrA, aadA2, qacEΔ1, sulI, orf5 | mph(A), mrx(A), mphR(A), blaTEM-1β catA | merA, merD, merE, merR |
| type II TA system: pemI/pemK (kis/kid, mazEF); | replication: repA2 (copB), repA1 (repA), copA | |||||||
Inc, incompatibility; Tra, Trb, conjugative transfer modules; mob, mobilization.
†
Regulatory genes involved in the control and effective functions including plasmid replication, copy number, incompatibility, and conjugative transfer. All homologs were predicted using BlastP.