Fig. 3.

Binding of trehalose-containing ligands to the CRD from human mincle. (A) Detection of trehalose dimycolate binding by glycolipid blotting. Glycolipids spotted on PVDF membrane were probed with biotin-tagged mincle CRD followed by alkaline phosphatase-conjugated avidin. (B) Quantification of trehalose binding to immobilized mincle CRD by competition with ¹²⁵I-labelled Man₃₁BSA. Experimental data are represented by dots and curves obtained by nonlinear least-squares fitting are shown as solid lines. Mean ± SD for the ratio of the K_I values derived from four replicate experiments is indicated.