Fig. 5.

Qdm-binding capability of T11D3. (A) Eu-based immunoassay to test the ability of folded T11 and T23 monomers of binding Qdm peptide. Folded MHC Ib monomers were captured on plates by the coated the anti-b2m mAb and incubated with the biotin-labeled peptides at room temperature overnight. The assay was set up in triplicates (n=3) and the experiment was repeated twice with similar results. One of them was shown. (B) and (C) Fluorescence polarization (FP) assay. Folded MHC Ib monomers were incubated with Alexfluro488 labeled Qdm peptides and the FP signal was recorded every 60 seconds at 37°C or 25°C, respectively. The experiments were repeated twice (37°C) and three times (25°C) with similar results. One of each was shown.