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. 2014 Oct 28;9(10):e110669. doi: 10.1371/journal.pone.0110669

Figure 5. Elucidation of role of tmAC in quality of sperm forward movement.

Figure 5

A. Effect of ddAdo (dideoxyadenosine) and db-cAMP (dibutyryl cAMP) in relation with FMSF-induced forward motility. B. Effect of ddAdo and KH7 in relation with FSK induced forward motility. Incubation periods with ddAdo, KH7, FSK and db-cAMP were of 30 min each. All the data represent mean ± SEM for n = 6 samples. Double asterisks (**) denote statistically significant difference vs. control (p<0.01), hash mark (#) indicates statistically significant inhibition vs. FMSF-treated (p<0.01), double hash mark (##) indicates statistically significant inhibition vs. control (p<0.01), asterisk (*) denotes statistically significant difference vs. (ddAdo+FMSF) (p<0.05), nsd denotes not significant difference vs. control. Viable cell count throughout the different assay conditions remained ≥95%. C. Effect of different doses of ddAdo on modulation of intracellular cAMP level in presence of FMSF (0.5 µM). Duration of ddAdo and FMSF incubation were 30 and 2 min, respectively. All the data represent mean ± SEM for n = 4 samples. Hash mark (##) indicates statistically significant inhibition vs. ddAdo-untreated (p<0.01).